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KMID : 0361020020450100963
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Korean Journal of Otolaryngology - Head and Neck Surgery
2002 Volume.45 No. 10 p.963 ~ p.968
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Expression of Collectin in the Human Nasal Mucosa
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황순재/Soon Jae Hwang
최진호/이상학/이진영/황유진*/이흥만/Jin Ho Choi/Sang Hag Lee/Jin Young Lee/You Jin Hwang*/Heung Man Lee
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Abstract
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Background and Objectives: Collectins (surfactant protein A and D) are proteins with collagen tails and globular lectin domains that appear to play an important role in the first line of host defense in mammalians. However, it is not known
if
collectins are also present in human nasal mucosa. The purpose of this study was to investigate the expression of collectin proteins in human nasal mucosa and to compare the expressions of SP-A and D mRNA in the normal nasal mucosa and in chronic
inflammatory nasal diseases.
Materials and Method: Ten chronic rhinosinusitis patients were recruited and ten normal nasal mucosae were used as normal controls. Reverse transcriptase-polymerase chain reaction was performed to detect SP-A and SP-D mRNA. The level of
collectin
and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) transcripts were semi-quantified with the desitometry. We have characterized the cellular localization of SP-A and SP-D protein using immunohistochemistry.
Results: SP-A2/GAPDH mRNA ratio in chronic rhinitis nasal mucosa is greater compared with that in normal nasal mucosa (p<0.05). SP-A protein was expressed in the nasal epithelium and in the epithelial cells of the submucosal glands. SP-D
mRNA and
protein were not expressed in the nasal mucosa.
Conclusion: These data provide the first evidence of the presence of collectins in the human nasal mucosa. These results suggested that up-regulation of collectin in chronic rhinosinusitis may be a protective response for the nasal mucosa.
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KEYWORD
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